课题名称 | 新疆猪源大肠杆菌耐药基因的流行性调查及其传播机制研究 Molecular Epidemiology of Resistance Genes among Escherichia coli from the Pigs and Molecular Mechanism of Transmission in Xinjiang | ||
执行时间 | 2013年1月1日-2016年12月30日 | 课题编号 | 31260614 |
项目来源 | 国家自然科学基金 | 资助经费 | 49.0万 |
主 持 人 | 夏利宁 | 结 题 否 | 否 |
成 员 | 包晓伟；郭庆勇；戴小华；王谨 | ||
摘 要 | 致病性大肠杆菌是人医和兽医临床感染中最常见的病原菌之一。大肠杆菌作为胃肠道中的共生菌，能够成为体内耐药性与耐药基因的“蓄水池”，其耐药性水平能够间接地表征、评估个体所暴露的耐药性风险。本课题针对新疆地区主要养猪场采集的大肠杆菌，通过微量稀释法获取菌株对三类常用抗生素的耐药信息，对耐药菌株进行喹诺酮类、氨基糖苷类和β-内酰胺类耐药基因检测，应用PFGE技术研究阳性菌株的亲缘关系，采用southern 杂交定位、质粒可转移性和不相容性分析、介导耐药基因转移的遗传元件结构图绘制及解析来探明其在大肠杆菌中传播的分子机制。上述研究能更全面的揭示多类耐药基因的流行传播机制，加深对养殖用药促进动物源耐药菌/耐药基因产生、传播的认识，为动物源耐药菌/耐药基因的转移扩散的风险评估和防控提供重要科学依据。 | ||
Abstract | Pathogenic E. coli is the most common pathogen in humans and animals. As the symbiotic bacteria in the gastrointestinal tract, E. coli can become a reservoir of the drug resistance and resistance genes. Therefore, resistance level of E.coli can indirectly indicate the risk of resistance, which individual is exposed. This project aims (1) to investigate the resistance information of E. coli, which will be collected from main pig farms in Xinjiang, to three common antibiotics, quinolones, aminoglycosides, and β-lactams, by the microdilution method; (2) to detect the resistance genes of resistant strains to the three antibiotics; (3) to determine the genetic relationship of positive strains by PFGE; and (4) to study the molecular mechanisms by which resistance is transferred in E. coli by southern hybridization, analysis of plasmid transferability and incompatibility, mapping and analysis of genetic elements which mediate the transfer of resistance genes. This project may reveal the epidemic dissemination mechanisms of multi-class resistance genes and improve the understanding that the drugs we used for animals may promote the production of resistant drugs as well as resistant genes. | ||